Open AccessmiRNA modulation of SOCS1 using an influenza A virus delivery system
Author(s) -
Leonard Izzard,
Siying Ye,
Kristie A. Jenkins,
Yingju Xia,
Mark Tizard,
John Stambas
Publication year - 2014
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/vir.0.063834-0
Subject(s) - gene knockdown , suppressor of cytokine signaling 1 , microrna , biology , luciferase , downregulation and upregulation , gene delivery , virology , influenza a virus , virus , delivery system , gene , transfection , microbiology and biotechnology , genetics , suppressor , pharmacology
Difficulties associated with efficient delivery and targeting of miRNAs to cells is hampering the real world application of miRNA technology. This study utilized an influenza A-based delivery system to express miR-155 in order to knockdown SOCS1 mRNA. Using qPCR and dual luciferase technology we show that miR-155 delivery resulted in a significant increase in cellular miR-155 which facilitated a downregulation of SOCS1 gene expression and a functional increase in IL-6 and IFN-β cytokines.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom