Open AccessCyclosporin A inhibits the replication of diverse coronaviruses
Author(s) -
Adriaan H. de Wilde,
Jessika C. Zevenhoven-Dobbe,
Yvonne van der Meer,
Volker Thiel,
Krishrayanan,
Shinji Makino,
Eric J. Snijder,
Martijn J. van Hemert
Publication year - 2011
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/vir.0.034983-0
Subject(s) - biology , coronavirus , virology , viral replication , coronaviridae , gene knockdown , virus , cytotoxic t cell , mouse hepatitis virus , cell culture , microbiology and biotechnology , covid-19 , genetics , in vitro , infectious disease (medical specialty) , medicine , disease , pathology
Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.
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