Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography | Zendy

Edwin J. B. Veldhuis Kroeze | Zendy; Geert van Amerongen | Zendy; Marcel L. Dijkshoorn | Zendy; James H. Simon | Zendy; Leon de Waal | Zendy; Ieneke J. C. Hartmann | Zendy; Gabriël P. Krestin | Zendy; Thijs Kuiken | Zendy; Albert D. M. E. Osterhaus | Zendy; Koert J. Stittelaar | Zendy

Open Access

Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography

Author(s) -

Edwin J. B. Veldhuis Kroeze,

Geert van Amerongen,

Marcel L. Dijkshoorn,

James H. Simon,

Leon de Waal,

Ieneke J. C. Hartmann,

Gabriël P. Krestin,

Thijs Kuiken,

Albert D. M. E. Osterhaus,

Koert J. Stittelaar

Publication year - 2011

Publication title -

journal of general virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.55

H-Index - 167

eISSN - 1465-2099

pISSN - 0022-1317

DOI - 10.1099/vir.0.032805-0

Subject(s) - histopathology , computed tomography , pathology , influenza a virus , pandemic , virus , biology , immunohistochemistry , lung , covid-19 , virology , medicine , radiology , infectious disease (medical specialty) , disease

We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies.

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