Open AccessThe complete genome sequence and proteomics of Yersinia pestis phage Yep-phi
Author(s) -
Xiangna Zhao,
Wei Wu,
Zhen Qi,
Yujun Cui,
Y. Yan,
Z. Guo,
Zhongdong Wang,
Hui Wang,
Haijun Deng,
Yan Xue,
Wei Chen,
Xiaolin Wang,
Ruifu Yang
Publication year - 2010
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/vir.0.026328-0
Subject(s) - genbank , biology , genome , genetics , orfs , lytic cycle , virology , sequence analysis , accession number (library science) , gene , bacteriophage , proteomics , open reading frame , peptide sequence , virus , escherichia coli
Yep-phi, a lytic phage of Yersinia pestis, was isolated in China and is routinely used as a diagnostic phage for the identification of the plague pathogen. Yep-phi has an isometric hexagonal head containing dsDNA and a short non-contractile conical tail. In this study, we sequenced the Yep-phi genome (GenBank accession no. HQ333270) and performed proteomics analysis. The genome consists of 38 ,616 bp of DNA, including direct terminal repeats of 222 bp, and is predicted to contain 45 ORFs. Most structural proteins were identified by proteomics analysis. Compared with the three available genome sequences of lytic phages for Y. pestis, the phages could be divided into two subgroups. Yep-phi displays marked homology to the bacteriophages Berlin (GenBank accession no. AM183667) and Yepe2 (GenBank accession no. EU734170), and these comprise one subgroup. The other subgroup is represented by bacteriophage ΦA1122 (GenBank accession no. AY247822). Potential recombination was detected among the Yep-phi subgroup.
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