Open AccessRole of annexin A2 in cellular entry of rabbit vesivirus
Author(s) -
Salomé GonzálezReyes,
Alberto García-Manso,
Gloria del Barrio,
Kevin P. Dalton,
Lorenzo González-Molleda,
José Arrojo-Fernández,
Inés Nicieza,
Francisco Parra
Publication year - 2009
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/vir.0.013276-0
Subject(s) - annexin a2 , internalization , biology , gene knockdown , hek 293 cells , virology , annexin , viral matrix protein , small interfering rna , virus , microbiology and biotechnology , cell culture , cell , transfection , genetics
The mechanisms of calicivirus attachment and internalization are not well understood, mainly due to the lack of a reliable cell-culture system for most of its members. In this study, rabbit vesivirus (RaV) virions were shown to bind annexin A2 (ANXA2) in a membrane protein fraction from HEK293T cells, using a virus overlay protein-binding assay and matrix-assisted laser desorption/ionization time-of-flight analysis. A monoclonal anti-ANXA2 antibody and small interfering RNA-mediated knockdown of ANXA2 expression in HEK293T cells reduced virus infection significantly, further supporting the role of ANXA2 in RaV attachment and/or internalization.
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