Open AccessDetection of exosomal prions in blood by immunochemistry techniques
Author(s) -
G. Properzi,
Emmanuel Comoy,
Mariantonia Logozzi,
Stefano Fais,
Ilaria Cristofaro,
Luana Lugini,
Massimo Venditti,
Hanin Abdel–Haq,
Maurizio Pocchiari,
Valérie Durand,
Daniela di Sevo,
Marisa Colone,
Franco Cardone,
Tommaso Azzarito,
Elena Ferroni,
Cristina Federici
Publication year - 2015
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/vir.0.000117
Subject(s) - immunochemistry , microvesicles , biology , infectivity , virology , antibody , exosome , immunology , virus , gene , microrna , biochemistry
In most forms of prion diseases, blood is infectious, but detection by immunochemistry techniques of the only available marker of infection (the misfolded prion protein, PrPTSE) in blood remains elusive. We developed a novel method for the detection of PrPTSE in blood of prion-infected rodents based on the finding that PrPTSE is associated with plasma exosomes. However, further purification of the exosomes on a sucrose gradient was necessary to remove plasma immunoglobulins, which interfere with PrPTSE, masking its detection by immunochemistry. Finally, we report that about 20% of plasma infectivity is associated with exosomes.
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