English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The ability of Salmonella enterica serovar Typhimurium to survive environmental stress requires specific, coordinated, responses, which induce resistance to the stress condition. This study investigated the relative contribution of sigmaE and sigmaS, the sigma factors regulating extracytoplasmic and general stress response functions, respectively, to survival at low temperature and also in media of differing osmotic strength, conditions relevant to food preservation. To determine if low-temperature storage is a signal for sigmaE- and sigmaS-mediated survival, the ability of S. Typhimurium rpoE, rpoS and rpoE/rpoS mutants to survive in a saline starvation-survival model at a refrigeration temperature (4.5 degrees C) was examined. Under these conditions, the rpoE mutant was significantly (P&lt;0.05) compromised compared to the parent and to an rpoS mutant. The double mutant in rpoE and rpoS displayed a cumulative defect in survival. In hyperosmotic environments (low aw) containing 6 % NaCl and at refrigeration temperature, both sigma factors were important for maximum survival but sigmaS played the dominant role. Analysis of the metabolic activity of starved populations at 4.5 and 37 degrees C revealed significantly (P&lt;0.001) elevated electron-transport system activity in mutants in rpoE and rpoS, indicating a role for sigmaE- and sigmaS-regulated genes in maintaining energy homeostasis. Together these data demonstrate that sigmaE and sigmaS are important for survival of S. Typhimurium in conditions encountered during food processing and that the relative contribution of sigmaE and sigmaS is critically dependent on the precise nature of the stress.

microbiology

Role of the alternative sigma factors σ E and σ S in survival of Salmonella enterica serovar Typhimurium during starvation, refrigeration and osmotic shock