Construction and characterization of a Lactococcus lactis strain deficient in intracellular ClpP and extracellular HtrA proteases | Zendy

Naima G. CortesPerez | Zendy; Isabelle Poquet | Zendy; Maria Auxiliadora Oliveira | Zendy; JeanJacques Gratadoux | Zendy; S M Madsen | Zendy; Anderson Miyoshi | Zendy; G. Corthier | Zendy; Vasco Azevedo | Zendy; Philippe Langella | Zendy; Luis G. BermúdezHumarán | Zendy

Open Access

Construction and characterization of a Lactococcus lactis strain deficient in intracellular ClpP and extracellular HtrA proteases

Author(s) -

Naima G. CortesPerez,

Isabelle Poquet,

Maria Auxiliadora Oliveira,

JeanJacques Gratadoux,

S M Madsen,

Anderson Miyoshi,

G. Corthier,

Vasco Azevedo,

Philippe Langella,

Luis G. BermúdezHumarán

Publication year - 2006

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.28698-0

Subject(s) - lactococcus lactis , heterologous , proteases , extracellular , heterologous expression , biology , microbiology and biotechnology , strain (injury) , mutant , context (archaeology) , secretion , nisin , intracellular , recombinant dna , biochemistry , enzyme , bacteria , gene , genetics , lactic acid , anatomy , paleontology , antimicrobial

A Lactococcus lactis strain deficient in both its major proteases, intracellular (ClpP) and extracellular (HtrA), was constructed and characterized. This strain, hereafter called clpP-htrA, could be obtained only by conjugation between a clpP donor strain and an htrA recipient strain in the NZ9000 context, allowing heterologous gene expression under the control of the NICE (nisin-controlled expression) system. The clpP-htrA double mutant showed both higher stress tolerance (e.g. high temperature and ethanol resistance) and higher viability than single clpP or htrA mutant strains. In addition, the secretion rate of two heterologous proteins (staphylococcal nuclease Nuc and Nuc-E7) was also higher in clpP-htrA than in the wild-type strain. This strain should be a useful host for high-level production and quality of stable heterologous proteins.

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