A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture | Zendy

Catherine M. O’Connell | Zendy; Kristy M. Nicks | Zendy

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A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture

Author(s) -

Catherine M. O’Connell,

Kristy M. Nicks

Publication year - 2006

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.28658-0

Subject(s) - plasmid , novobiocin , strain (injury) , biology , microbiology and biotechnology , infectivity , phenotype , chlamydia , chlamydia trachomatis , gene , genetics , virology , virus , anatomy , antibiotics

A highly conserved cryptic plasmid is present in Chlamydia trachomatis yet naturally occurring plasmid-deficient isolates are very rare. This paper describes the isolation and characterization of a plasmid-deficient strain of C. muridarum, using novobiocin as a curing agent. Plasmid-deficient derivatives of C. muridarum strain Nigg were generated at high efficiencies (4-30%). Phenotypic characterization revealed that the cured derivative was unable to accumulate glycogen within intracytoplasmic inclusions. In addition, this strain formed small plaques at a reduced efficiency compared to the wild-type parent.

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