Characterization of the Bacillus cereus Nhe enterotoxin | Zendy

Toril Lindbäck | Zendy

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Characterization of the Bacillus cereus Nhe enterotoxin

Author(s) -

Toril Lindbäck

Publication year - 2004

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.27359-0

Subject(s) - enterotoxin , bacillus cereus , cereus , escherichia coli , bacillus subtilis , operon , microbiology and biotechnology , biology , histidine , bacillaceae , gene , chemistry , two component regulatory system , biochemistry , bacteria , amino acid , genetics , mutant

The non-haemolytic enterotoxin (Nhe) is one of two three-component enterotoxins responsible for the diarrhoeal food-poisoning syndrome caused by Bacillus cereus. Nhe is composed of NheA, NheB and NheC. The three genes encoding the Nhe components constitute an operon, and the transcriptional start site is located 61 bp upstream of the nheA translational start site. The nhe genes were cloned separately, and expressed in either Bacillus subtilis or Escherichia coli. Separate expression showed that all three components were required for biological activity. In addition, NheA and NheB were purified from B. cereus culture supernatants. As NheC seems to be expressed in only small amounts by B. cereus, NheC was expressed and purified as a histidine-tagged fusion protein. The maximum cytotoxic activity was obtained when the molar ratio between NheA : NheB : His6-NheC was 10 : 10 : 1, and it was shown that NheB was the binding component of the enterotoxin complex.

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