Role of Pir1 in the construction of the Candida albicans cell wall | Zendy

Ana I. Martı́nez | Zendy

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Role of Pir1 in the construction of the Candida albicans cell wall

Author(s) -

Ana I. Martı́nez

Publication year - 2004

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.27220-0

Subject(s) - candida albicans , saccharomyces cerevisiae , mutant , biology , homology (biology) , cell wall , corpus albicans , gene , epitope , dna , microbiology and biotechnology , amino acid , genetics , antibody

Searches in a Candida albicans database (http://genolist.pasteur.fr/CandidaDB/) identified two Individual Protein Files (IPF 15363 and 19968) whose deduced amino acid sequences showed 42 % and 45 % homology with Saccharomyces cerevisiae Pir4. The two DNA sequences are alleles of the same gene (CaPIR1) but IPF 19968 has a deletion of 117 bases. IPF 19968 encodes a putative polypeptide of 364 aa, which is highly O-glycosylated and has an N-mannosylated chain, four cysteine residues and seven repeats. Both alleles are expressed under different growth conditions and during wall construction by regenerating protoplasts. The heterozygous mutant cells are elongated, form clumps of several cells and are hypersensitive to drugs that affect cell wall assembly. CaPir1 was labelled with the V5 epitope and found linked to the 1,3-beta-glucan of the C. albicans wall and also by disulphide bridges when expressed in S. cerevisiae.

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