The phrA gene of Rhodobacter sphaeroides encodes a photolyase and is regulated by singlet oxygen and peroxide in a σ E-dependent manner | Zendy

Anne-Kathrin Hendrischk | Zendy; Stephan Braatsch | Zendy; Jens Glaeser | Zendy; Gabriele Klug | Zendy

Open Access

The phrA gene of Rhodobacter sphaeroides encodes a photolyase and is regulated by singlet oxygen and peroxide in a σ E-dependent manner

Author(s) -

Anne-Kathrin Hendrischk,

Stephan Braatsch,

Jens Glaeser,

Gabriele Klug

Publication year - 2007

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.2006/004390-0

Subject(s) - rhodobacter sphaeroides , photolyase , sigma factor , singlet oxygen , cryptochrome , hydrogen peroxide , photochemistry , biology , gene , photosynthesis , chemistry , biochemistry , gene expression , dna repair , oxygen , promoter , circadian clock , organic chemistry

The genome of the facultatively photosynthetic bacterium Rhodobacter sphaeroides encodes three proteins of the photolyase/cryptochrome family. This paper shows that phrA (RSP2143) encodes a functional photolyase, which is an enzyme that repairs UV radiation-induced DNA damage in a blue light dependent manner. Expression of phrA is upregulated in response to light, with no photoreceptor or the photosynthetic electron transport being involved. The results reveal that singlet oxygen and hydrogen peroxide dependent signals are transmitted by the sigma(E) factor and the anti-sigma(E) factor ChrR affecting phrA expression, while superoxide anions do not stimulate phrA expression. Thus, the sigma(E) regulon is involved not only in the response to singlet oxygen but also in the hydrogen peroxide response.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research