Open AccessExtracellular carbonic anhydrases of the stromatolite-forming cyanobacterium Microcoleus chthonoplastes
Author(s) -
Elena V. Kupriyanova,
Arsenio Villarejo,
Alexandra G. Markelova,
Л. М. Герасименко,
Georgy A. Zavarzin,
Göran Samuelsson,
Dmitry A. Los,
Н. А. Пронина
Publication year - 2007
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/mic.0.2006/003905-0
Subject(s) - extracellular , stromatolite , biochemistry , western blot , cyanobacteria , chemistry , extracellular matrix , biology , bacteria , gene , genetics , organic chemistry , carbonate
Active extracellular carbonic anhydrases (CAs) were found in the alkaliphilic stromatolite-forming cyanobacterium Microcoleus chthonoplastes. Enzyme activity was detected in intact cells and in the cell envelope fraction. Western blot analysis of polypeptides from the cell envelope suggested the presence of at least two polypeptides cross-reacting with antibodies against both alpha and beta classes of CA. Immunocytochemical analysis revealed putative alpha-CA localized in the glycocalyx. This alpha-CA has a molecular mass of about 34 kDa and a pI of 3.5. External CAs showed two peaks of activity at around pH 10 and 7.5. The possible involvement of extracellular CAs of M. chthonoplastes in photosynthetic assimilation of inorganic carbon and its relationship to CaCO(3) deposition during mineralization of cyanobacterial cells are discussed.
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