Rhizobium tropici response to acidity involves activation of glutathione synthesis | Zendy

Cecilia Isabel Muglia | Zendy; Daniel H. Grasso | Zendy; O. Mario Aguilar | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Rhizobium tropici response to acidity involves activation of glutathione synthesis

Author(s) -

Cecilia Isabel Muglia,

Daniel H. Grasso,

O. Mario Aguilar

Publication year - 2007

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.2006/003483-0

Subject(s) - phaseolus , mutant , gene , biology , transcription (linguistics) , biochemistry , glutathione , gene expression , strain (injury) , rhizobium , microbiology and biotechnology , chemistry , enzyme , botany , linguistics , philosophy , anatomy

Rhizobium tropici CIAT899 displays intrinsic tolerance to acidity, and efficiently nodulates Phaseolus vulgaris at low pH. By characterizing a gshB mutant strain, glutathione has been previously demonstrated to be essential for R. tropici tolerance to acid stress. The wild-type gshB gene region has been cloned and its transcription profile has been characterized by using quantitative real-time PCR and transcriptional gene fusions. Activation of the gshB gene under acid-stress conditions was demonstrated. gshB is also induced by UV irradiation. Upstream from gshB a putative sigma(70) promoter element and an inverted repeat sequence were identified, which are proposed to be involved in expression under neutral and acidic conditions, respectively. Gel retardation assays indicate that transcription in acid conditions may involve protein binding to an upstream regulatory region.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research