Identification of membrane-bound quinoprotein inositol dehydrogenase in Gluconobacter oxydans ATCC 621H | Zendy

Tina Hölscher | Zendy; D. Weinert-Sepalage | Zendy; Helmut Görisch | Zendy

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Identification of membrane-bound quinoprotein inositol dehydrogenase in Gluconobacter oxydans ATCC 621H

Author(s) -

Tina Hölscher,

D. Weinert-Sepalage,

Helmut Görisch

Publication year - 2007

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.2006/002196-0

Subject(s) - pyrroloquinoline quinone , dehydrogenase , biochemistry , inositol , cofactor , mutant , chemistry , biology , enzyme , gene , receptor

The GOX1857 gene, which encodes a putative membrane-bound pyrroloquinoline quinone (PQQ)-dependent dehydrogenase in Gluconobacter oxydans ATCC 621H, was characterized. GOX1857 was disrupted and the oxidizing potential of the resulting mutant strain was compared to that of the wild-type. In contrast to the wild-type, the mutant was unable to grow with myo-inositol as the sole energy source and did not show any myo-inositol dehydrogenase activity in vitro, indicating that GOX1857 encodes an inositol dehydrogenase. The association of inositol dehydrogenase with the membrane and the requirement for the cofactor PQQ were confirmed. Inositol dehydrogenase exhibited optimal activity at pH 8.75. As indicated by cultivation on different substrates, inositol dehydrogenase was repressed by d-glucose.

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