UlaR activates expression of the ula operon in Streptococcus pneumoniae in the presence of ascorbic acid | Zendy

Muhammad Afzal | Zendy; Sulman Shafeeq | Zendy; Birgitta HenriquesNormark | Zendy; Oscar P. Kuipers | Zendy

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UlaR activates expression of the ula operon in Streptococcus pneumoniae in the presence of ascorbic acid

Author(s) -

Muhammad Afzal,

Sulman Shafeeq,

Birgitta HenriquesNormark,

Oscar P. Kuipers

Publication year - 2014

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.083899-0

Subject(s) - operon , ascorbic acid , lac operon , streptococcus pneumoniae , transcriptional regulation , gene , biology , regulator , biochemistry , chemistry , genetics , gene expression , bacteria , mutant , food science

In this study, the regulatory mechanism of the ula (utilization of l-ascorbic acid) operon, putatively responsible for transport and utilization of ascorbic acid in Streptococcus pneumoniae strain D39, is studied. β-Galactosidase assay data demonstrate that expression of the ula operon is increased in the presence of ascorbic acid as compared with the effects of other sugar sources including glucose. The ula operon consists of nine genes, including a transcriptional regulator UlaR, and is transcribed as a single transcriptional unit. We demonstrate the role of the transcriptional regulator UlaR as a transcriptional activator of the ula operon in the presence of ascorbic acid and show that activation of the ula operon genes by UlaR is CcpA-independent. Furthermore, we predict a 16 bp regulatory site (5'-AACAGTCCGCTGTGTA-3') for UlaR in the promoter region of ulaA. Deletion of the half or full UlaR regulatory site in PulaA confirmed that the UlaR regulatory site present in PulaA is functional.

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