Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage | Zendy

Dong Hwan Lee | Zendy; Jeong-A Lim | Zendy; Juneok Lee | Zendy; Eunjung Roh | Zendy; Kyu-Suk Jung | Zendy; MinSeon Choi | Zendy; ChangSik Oh | Zendy; Sangryeol Ryu | Zendy; Jong-Chul Yun | Zendy; Sunggi Heu | Zendy

Open Access

Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage

Author(s) -

Dong Hwan Lee,

Jeong-A Lim,

Juneok Lee,

Eunjung Roh,

Kyu-Suk Jung,

MinSeon Choi,

ChangSik Oh,

Sangryeol Ryu,

Jong-Chul Yun,

Sunggi Heu

Publication year - 2013

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.067280-0

Subject(s) - pectobacterium carotovorum , biology , virulence , transposon mutagenesis , microbiology and biotechnology , mutant , pectobacterium , gene , pathogen , biofilm , genetics , mutagenesis , transposable element , bacteria

Pectobacterium carotovorum subsp. carotovorum is a well-known plant pathogen that causes severe soft rot disease in various crops, resulting in considerable economic loss. To identify pathogenicity-related factors, Chinese cabbage was inoculated with 5314 transposon mutants of P. carotovorum subsp. carotovorum Pcc21 derived using Tn5 transposon mutagenesis. A total of 35 reduced-virulence or avirulent mutants were isolated, and 14 loci were identified. The 14 loci could be functionally grouped into nutrient utilization (pyrD, purH, purD, leuA and serB), production of plant cell-wall-degrading enzymes (PCWDEs) (expI, expR and PCC21_023220), motility (flgA, fliA and flhB), biofilm formation (expI, expR and qseC), susceptibility to antibacterial plant chemicals (tolC) and unknown function (ECA2640). Among the 14 genes identified, qseC, tolC and PCC21_023220 are novel pathogenicity factors of P. carotovorum subsp. carotovorum involved in biofilm formation, phytochemical resistance and PCWDE production, respectively.

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