Interaction of Rickettsia felis with histone H2B facilitates the infection of a tick cell line | Zendy

Chutima Thepparit | Zendy; Apichai Bourchookarn | Zendy; Natthida Petchampai | Zendy; Steven A. Barker | Zendy; Kevin R. Macaluso | Zendy

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Interaction of Rickettsia felis with histone H2B facilitates the infection of a tick cell line

Author(s) -

Chutima Thepparit,

Apichai Bourchookarn,

Natthida Petchampai,

Steven A. Barker,

Kevin R. Macaluso

Publication year - 2010

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.041400-0

Subject(s) - biology , felis , rickettsia , histone h2b , virology , tick , histone , microbiology and biotechnology , genetics , cats , dna , medicine , virus

Haematophagous arthropods are the primary vectors in the transmission of Rickettsia, yet the molecular mechanisms mediating the rickettsial infection of arthropods remain elusive. This study utilized a biotinylated protein pull-down assay together with LC-MS/MS to identify interaction between Ixodes scapularis histone H2B and Rickettsia felis. Co-immunoprecipitation of histone with rickettsial cell lysate demonstrated the association of H2B with R. felis proteins, including outer-membrane protein B (OmpB), a major rickettsial adhesin molecule. The rickettsial infection of tick ISE6 cells was reduced by approximately 25 % via RNA-mediated H2B-depletion or enzymic treatment of histones. The interaction of H2B with the rickettsial adhesin OmpB suggests a role for H2B in mediating R. felis internalization into ISE6 cells.

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