The endolysins of bacteriophages CMP1 and CN77 are specific for the lysis of Clavibacter michiganensis strains | Zendy

Johannes Wittmann | Zendy; Rudolf Eichenlaub | Zendy; Brigitte Dreiseikelmann | Zendy

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The endolysins of bacteriophages CMP1 and CN77 are specific for the lysis of Clavibacter michiganensis strains

Author(s) -

Johannes Wittmann,

Rudolf Eichenlaub,

Brigitte Dreiseikelmann

Publication year - 2010

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.037291-0

Subject(s) - clavibacter michiganensis , lysin , biology , microbiology and biotechnology , bacteria , molecular mass , escherichia coli , lysis , bacteriophage , enzyme , biochemistry , gene , pathogen , genetics

Putative endolysin genes of bacteriophages CMP1 and CN77, which infect Clavibacter michiganensis subsp. michiganensis and C. michiganensis subsp. nebraskensis, respectively, were cloned and expressed in Escherichia coli. The His-tagged endolysin of CMP1 consists of 306 amino acids and has a calculated molecular mass of 34.8 kDa, while the His-tagged endolysin of CN77 has 290 amino acids with a molecular mass of 31.9 kDa. The proteins were purified and their bacteriolytic activity was demonstrated. The bacteriolytic activity of both enzymes showed a host range which was limited to the respective C. michiganensis subspecies and did not affect other bacteria, even those closely related to Clavibacter. Due to the high specificity of the CMP1 and CN77 endolysins they may be useful tools for biocontrol of plant-pathogenic C. michiganensis without affecting other bacteria in the soil.

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