Characterization of a broad-host-range flagellum-dependent phage that mediates high-efficiency generalized transduction in, and between, Serratia and Pantoea | Zendy

Terry John Evans | Zendy; M. A. Crow | Zendy; Neil R. Williamson | Zendy; Wendy Orme | Zendy; Nicholas R. Thomson | Zendy; E. Komitopoulou | Zendy; George P. C. Salmond | Zendy

Open Access

Characterization of a broad-host-range flagellum-dependent phage that mediates high-efficiency generalized transduction in, and between, Serratia and Pantoea

Author(s) -

Terry John Evans,

M. A. Crow,

Neil R. Williamson,

Wendy Orme,

Nicholas R. Thomson,

E. Komitopoulou,

George P. C. Salmond

Publication year - 2009

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.032797-0

Subject(s) - serratia , biology , microbiology and biotechnology , transduction (biophysics) , pantoea agglomerans , plasmid , bacteria , flagellum , serratia marcescens , bacteriophage , cosmid , gene , genetics , botany , escherichia coli , pseudomonas

A phage (PhiOT8) isolated on Serratia sp. ATCC 39006 was shown to be flagellum-dependent, and to mediate generalized transduction with high efficiency (up to 10(-4) transductants per p.f.u.). PhiOT8 was shown to have a broad host range because it also infected a strain of Pantoea agglomerans isolated from the rhizosphere. Transduction of plasmid-borne antibiotic resistance between the two bacterial genera was demonstrated, consistent with purported ecological roles of phages in dissemination of genes between bacterial genera. Serratia sp. ATCC 39006 and P. agglomerans produce a number of interesting secondary metabolites that have potential applications in cancer therapy and biocontrol of fungal infections. PhiOT8 has utility as a powerful functional genomics tool in these bacteria.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research