Gene cloning and characteristics of the RND-type multidrug efflux pump MuxABC-OpmB possessing two RND components in Pseudomonas aeruginosa | Zendy

Takehiko Mima | Zendy; Naoki Kohira | Zendy; Yang Li | Zendy; Hiroshi Sekiya | Zendy; Wakano Ogawa | Zendy; Teruo Kuroda | Zendy; Tomofusa Tsuchiya | Zendy

Open Access

Gene cloning and characteristics of the RND-type multidrug efflux pump MuxABC-OpmB possessing two RND components in Pseudomonas aeruginosa

Author(s) -

Takehiko Mima,

Naoki Kohira,

Yang Li,

Hiroshi Sekiya,

Wakano Ogawa,

Teruo Kuroda,

Tomofusa Tsuchiya

Publication year - 2009

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.019

H-Index - 179

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.031260-0

Subject(s) - efflux , novobiocin , pseudomonas aeruginosa , biology , microbiology and biotechnology , multiple drug resistance , mutant , tetracycline , plasmid , genetics , gene , drug resistance , antibiotics , bacteria

muxA-muxB-muxC-opmB (formerly PA2528-PA2527-PA2526-opmB), encoding a putative resistance nodulation cell division (RND)-type multidrug efflux pump system, was cloned from Pseudomonas aeruginosa PAO1. Introduction of muxABC-opmB into P. aeruginosa YM64, a drug-hypersusceptible strain, led to elevated MICs of aztreonam, macrolides, novobiocin and tetracycline. Since muxB and muxC, both of which encode RND components, were essential for function, MuxABC-OpmB is thought to be a drug efflux pump with four components. One novobiocin-resistant mutant, PMX725, isolated from P. aeruginosa PMX7 showed elevated resistance not only to novobiocin but also to aztreonam, macrolides and tetracycline. Increased mRNA expression of muxABC-opmB was observed in the mutant PMX725 compared with the parental strain. Sequencing analysis revealed that a single-nucleotide insertion had occurred in the deduced promoter region for muxABC-opmB in PMX725. In this study, we have characterized the last RND-type multidrug efflux pump predicted from the genome sequence in P. aeruginosa.

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