Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae | Zendy

Rachida Mersni-Achour | Zendy; Yosra Ben Cheikh | Zendy; Vianney Pichereau | Zendy; Ibtissem Doghri | Zendy; Cédric Etien | Zendy; Lionel Dégremont | Zendy; Denis Saulnier | Zendy; Ingrid FruitierArnaudin | Zendy; MarieAgnès Travers | Zendy

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Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae

Author(s) -

Rachida Mersni-Achour,

Yosra Ben Cheikh,

Vianney Pichereau,

Ibtissem Doghri,

Cédric Etien,

Lionel Dégremont,

Denis Saulnier,

Ingrid FruitierArnaudin,

MarieAgnès Travers

Publication year - 2015

Publication title -

microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.352

H-Index - 35

eISSN - 1465-2080

pISSN - 1350-0872

DOI - 10.1099/mic.0.000058

Subject(s) - crassostrea , virulence , biology , microbiology and biotechnology , pacific oyster , oyster , metalloproteinase , larva , extracellular , shellfish , vibrio , vibrionaceae , aquatic animal , bacteria , fishery , enzyme , ecology , biochemistry , fish <actinopterygii> , gene , genetics

Vibrio tubiashii is a marine pathogen isolated from larval and juvenile bivalve molluscs that causes bacillary necrosis. Recent studies demonstrated the isolation of this species in a French experimental hatchery/nursery affecting Crassostrea gigas spat in 2007. Here, using larvae of C. gigas as an interaction model, we showed that the French V. tubiashii is virulent to larvae and can cause bacillary necrosis symptoms with an LD50 of about 2.3 × 10(3) c.f.u. ml(-1) after 24 h. Moreover, complete or gel permeation HPLC fractionated extracellular products (ECPs) of this strain appeared toxic to larvae. MS-MS analysis of the different ECP fractions revealed the existence of an extracellular metalloprotease and other suspected virulence factors. This observation is also supported by the expression level of some potential virulence factors. The overall results suggest that the pathology caused by the French V. tubiashii in C. gigas oysters is caused by a group of toxic factors and not only the metalloprotease.

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