Methionine-mediated gene expression and characterization of the CmhR regulon in Streptococcus pneumoniae | Zendy

Muhammad Afzal | Zendy; Sulman Shafeeq | Zendy; Oscar P. Kuipers | Zendy

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Methionine-mediated gene expression and characterization of the CmhR regulon in Streptococcus pneumoniae

Author(s) -

Muhammad Afzal,

Sulman Shafeeq,

Oscar P. Kuipers

Publication year - 2016

Publication title -

microbial genomics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.476

H-Index - 28

ISSN - 2057-5858

DOI - 10.1099/mgen.0.000091

Subject(s) - regulon , methionine , gene , streptococcus pneumoniae , operon , transcriptome , biology , microbiology and biotechnology , promoter , transcriptional regulation , activator (genetics) , gene expression , genetics , chemistry , amino acid , bacteria , mutant

This study investigated the transcriptomic response of Streptococcus pneumoniae D39 to methionine. Transcriptome comparison of the S. pneumoniae D39 wild-type grown in chemically defined medium with 0–10 mM methionine revealed the elevated expression of various genes/operons involved in methionine synthesis and transport ( fhs , folD , gshT , metA , metB-csd , metEF , metQ , tcyB , spd-0150 , spd-0431 and spd-0618 ). Furthermore, β -galactosidase assays and quantitative RT-PCR studies demonstrated that the transcriptional regulator, CmhR (SPD-0588), acts as a transcriptional activator of the fhs , folD , metB-csd , metEF , metQ and spd-0431 genes. A putative regulatory site of CmhR was identified in the promoter region of CmhR-regulated genes and this CmhR site was further confirmed by promoter mutational experiments.

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