Intraspecific mitochondrial DNA polymorphism within the emerging filamentous fungal pathogen Trichoderma longibrachiatum | Zendy

Zsuzsanna Antal | Zendy; János Varga | Zendy; László Kredics | Zendy; András Szekeres | Zendy; Lóránt Hatvani | Zendy; László Manczinger | Zendy; Csaba Vágvölgyi | Zendy; Elisabeth Nagy | Zendy

Open Access

Intraspecific mitochondrial DNA polymorphism within the emerging filamentous fungal pathogen Trichoderma longibrachiatum

Author(s) -

Zsuzsanna Antal,

János Varga,

László Kredics,

András Szekeres,

Lóránt Hatvani,

László Manczinger,

Csaba Vágvölgyi,

Elisabeth Nagy

Publication year - 2005

Publication title -

journal of medical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.91

H-Index - 117

eISSN - 1473-5644

pISSN - 0022-2615

DOI - 10.1099/jmm.0.46292-0

Subject(s) - biology , mitochondrial dna , internal transcribed spacer , genetic diversity , pathogen , trichoderma , genetics , restriction fragment length polymorphism , intraspecific competition , microbiology and biotechnology , dendrogram , genetic variation , restriction enzyme , polymerase chain reaction , dna , gene , botany , ribosomal rna , zoology , population , demography , sociology

The genetic diversity of the emerging fungal pathogen Trichoderma longibrachiatum was examined at the level of mitochondrial DNA. The 17 investigated strains, comprising nine clinical and eight non-clinical isolates, exhibited seven and ten different mitochondrial DNA profiles by using the restriction enzymes BsuRI and Hin6I, respectively. The sizes of mitochondrial DNAs varied from 34.9 to 39.5 kb. The discriminatory power of the method was higher than that of internal transcribed spacer sequence analysis and therefore should be more suitable for identification and epidemiological investigations. However, clinical and non-clinical isolates did not form separate clusters on the resulting dendrogram and thus there was no indication of a correlation between genetic structure and pathogenicity of the isolates.

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