Open AccessA novel method for simple detection of mutations conferring drug resistance in Mycobacterium leprae, based on a DNA microarray, and its applicability in developing countries
Author(s) -
Masao Matsuoka,
Khin Saw Aye,
Kyaw Kyaw,
Esterlina V. Tan,
Ma. Victoria F. Balagon,
Paul Saunderson,
Robert H. Gelber,
Masanao Makino,
Chie Nakajima,
Yasuhiko Suzuki
Publication year - 2008
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.2008/002600-0
Subject(s) - mycobacterium leprae , rpob , leprosy , dapsone , drug resistance , biology , ofloxacin , dna sequencing , dna microarray , mutation , microarray , computational biology , genetics , gene , antibiotics , immunology , ciprofloxacin , gene expression , 16s ribosomal rna
A simple method to detect mutations in the genome of Mycobacterium leprae that confer resistance to key drugs for leprosy was exploited on the basis of a reverse hybridization system. A series of oligonucleotide probes corresponding to each mutation in the folP1, rpoB and gyrA genes for dapsone, rifampicin and ofloxacin resistance, respectively, were selected and fixed on a glass slide as capture probes, to develop a DNA microarray termed the leprosy drug susceptibility-DNA microarray (LDS-DA). Mutations in clinical isolates of M. leprae were successfully identified by the LDS-DA. Feasibility studies were conducted to evaluate the performance of the LDS-DA in two developing countries, Myanmar and the Philippines. The high concordance of the results obtained by this method with the results of nucleotide sequencing strongly supports the applicability of the LDS-DA as a drug susceptibility test in place of sequencing, a time-consuming and costly procedure. This is a rapid and simple method for the simultaneous susceptibility testing of three front-line drugs for leprosy, and solves the problems of previously reported methods.