Detection and quantification of Erysipelothrix rhusiopathiae in blood from infected chickens – addressing challenges with detection of DNA from infectious agents in host species with nucleated red blood cells | Zendy

Eva Wattrang | Zendy; Victoria Jäderblom | Zendy; Tomas Jinnerot | Zendy; Helena Eriksson | Zendy; Elisabeth Bagge | Zendy; M. Persson | Zendy; Tina Sørensen Dalgaard | Zendy; Robert Söderlund | Zendy

Open Access

Detection and quantification of Erysipelothrix rhusiopathiae in blood from infected chickens – addressing challenges with detection of DNA from infectious agents in host species with nucleated red blood cells

Author(s) -

Eva Wattrang,

Victoria Jäderblom,

Tomas Jinnerot,

Helena Eriksson,

Elisabeth Bagge,

M. Persson,

Tina Sørensen Dalgaard,

Robert Söderlund

Publication year - 2019

Publication title -

journal of medical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.91

H-Index - 117

eISSN - 1473-5644

pISSN - 0022-2615

DOI - 10.1099/jmm.0.001016

Subject(s) - dna extraction , biology , centrifugation , microbiology and biotechnology , whole blood , bacteria , polymerase chain reaction , immunomagnetic separation , chromatography , real time polymerase chain reaction , chemistry , immunology , genetics , gene , biochemistry

The present study aimed to establish pretreatment protocols as well as real-time and droplet digital polymerase chain reaction (PCR) methodologies to detect and quantify Erysipelothrix rhusiopathiae (ER) DNA in blood samples from infected chickens, as tools for routine diagnostics and monitoring of experimental infections. Chicken blood is a problematic matrix for PCR analysis because nucleated erythrocytes contribute large amounts of host DNA that inhibit amplification.

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