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The present study aimed to establish pretreatment protocols as well as real-time and droplet digital polymerase chain reaction (PCR) methodologies to detect and quantify Erysipelothrix rhusiopathiae (ER) DNA in blood samples from infected chickens, as tools for routine diagnostics and monitoring of experimental infections. Chicken blood is a problematic matrix for PCR analysis because nucleated erythrocytes contribute large amounts of host DNA that inhibit amplification.

journal of medical microbiology/journal of medical microbiology

Detection and quantification of Erysipelothrix rhusiopathiae in blood from infected chickens – addressing challenges with detection of DNA from infectious agents in host species with nucleated red blood cells