DNA extraction from primary liquid blood cultures for bloodstream infection diagnosis using whole genome sequencing | Zendy

Luke Anson | Zendy; Kevin Chau | Zendy; Nicholas Sanderson | Zendy; Sarah Hoosdally | Zendy; Phelim Bradley | Zendy; Zamin Iqbal | Zendy; Hang Phan | Zendy; Dona Foster | Zendy; Sarah Oakley | Zendy; Marcus Morgan | Zendy; Tim Peto | Zendy; Derrick W. Crook | Zendy; Louise Pankhurst | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

DNA extraction from primary liquid blood cultures for bloodstream infection diagnosis using whole genome sequencing

Author(s) -

Luke Anson,

Kevin Chau,

Nicholas Sanderson,

Sarah Hoosdally,

Phelim Bradley,

Zamin Iqbal,

Hang Phan,

Dona Foster,

Sarah Oakley,

Marcus Morgan,

Tim Peto,

Derrick W. Crook,

Louise Pankhurst

Publication year - 2018

Publication title -

journal of medical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.91

H-Index - 117

eISSN - 1473-5644

pISSN - 0022-2615

DOI - 10.1099/jmm.0.000664

Subject(s) - minion , dna extraction , biology , lysis , differential centrifugation , dna , whole genome sequencing , blood culture , microbiology and biotechnology , bacteria , pathogen , dna sequencing , centrifugation , genome , antibiotics , polymerase chain reaction , gene , genetics , nanopore sequencing , biochemistry

Speed of bloodstream infection diagnosis is vital to reduce morbidity and mortality. Whole genome sequencing (WGS) performed directly from liquid blood culture could provide single-assay species and antibiotic susceptibility prediction; however, high inhibitor and human cell/DNA concentrations limit pathogen recovery. We develop a method for the preparation of bacterial DNA for WGS-based diagnostics direct from liquid blood culture.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research