Evaluation of two commercial real-time PCR assays for detection of carbapenemase genes in Enterobacteriaceae | Zendy

Liat Ashkenazi Hoffnung | Zendy; Ela O Burdelova | Zendy; Amos Adler | Zendy

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Evaluation of two commercial real-time PCR assays for detection of carbapenemase genes in Enterobacteriaceae

Author(s) -

Liat Ashkenazi Hoffnung,

Ela O Burdelova,

Amos Adler

Publication year - 2017

Publication title -

journal of medical microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.91

H-Index - 117

eISSN - 1473-5644

pISSN - 0022-2615

DOI - 10.1099/jmm.0.000618

Subject(s) - enterobacteriaceae , biology , microbiology and biotechnology , carbapenem resistant enterobacteriaceae , gene , real time polymerase chain reaction , polymerase chain reaction , genetics , escherichia coli

The implementation of PCR for the detection of carbapenemase genes enables rapid results with significant epidemiological implications. Two commercial real-time PCR assays, the Hylabs Hy-CRE and Sacace MDR MBL+KPC/OXA, were evaluated for the detection of the genes blaKPC, blaVIM, blaNDM, blaIMP and blaOXA-48-carbapenemasein a collection of 96 carbapenem-resistant Enterobacteriaceae strains with different resistant mechanisms. Both assays exhibited excellent diagnostic performance, with 100 % sensitivity and specificity.

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