Rubneribacter badeniensis gen. nov., sp. nov. and Enteroscipio rubneri gen. nov., sp. nov., new members of the Eggerthellaceae isolated from human faeces

Two novel, anaerobic, Gram-positive, rod-shaped bacterial strains, ResAG-85 T and ResAG-96 T , were isolated from a faecal sample of a male human. 16S rRNA gene sequences analyses indicated that these strains represent a distinct lineage within the family Eggerthellaceae. Strain ResAG-85 T showed 92.3 % similarity to the type strains of the genera Eggerthella and Gordonibacter. Strain ResAG-96 T clustered together with Paraeggerthella hongkongensis and the newly (but not validly) published genus 'Arabia massiliensis' (94.8 % similarity). Analysis of quinones revealed that MK-5 (21 % in ResAG-85 T and 95 % in ResAG-96 T ) and MK-7 (53 % in strain ResAG-85 T ) were present, which were described for the first time for members of the Eggerthellaceae. Furthermore, MK-6 was present in both strains (25 % ResAG-85 T and 5 % in ResAG-96 T ). The polar lipids detected in ResAG-85 T and ResAG-96 T consisted of eight and six glycolipids, respectively. Both strains possessed three phospholipids, one phosphatidylglycerol and one diphosphatidylglycerol. Analysis of fatty acids revealed that the percentage of total branched fatty acids was relatively high in comparison to related strains with 42 and 50 % of strains ResAG-85 T and ResAG-96 T but comparable to the value obtained for Gordonibacter pamelaeae DSM 19378 T . On the basis of this polyphasic approach including molecular (16S rRNA gene sequencing) and biochemical methods (analysis of fatty acids, quinones, polar lipids, Rapid ID 32A and API 20A), the new genera and species Rubneribacter badeniensis with ResAG-85 T (=DSM 105129 T =JCM 32272 T ) and Enteroscipio rubneri with ResAG-96 T (=DSM 105130 T =JCM 32273 T ) as the type and only strains are described.