Pseudonocardia soli sp. nov., isolated from mountain soil

A novel actinomycete, designated strain NW8-21 T , was isolated from mountain soil in Mae-Wong National Park, Nakornsawan province, Thailand, and was taxonomically characterized by using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the strain was revealed to have the closest similarity to Pseudonocardia endophytica YIM 56035 T with the highest 16S rRNA gene sequence similarity value of 98.7 %, followed by Pseudonocardia nantongensis KLBMP 1282 T (98.0 %). The chemotaxonomic properties, i.e. arabinose and galactose as the diagnostic reducing sugar in cells, MK-8 (H4) as a major menaquinone, iso-C16 : 0 as the main cellular fatty acid component and phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol, phosphatidylcholine as the characteristic phospholipids, confirmed a taxonomic affiliation of the strain that was consistent with those of the genus Pseudonocardia. Several phenotypic differences and the DNA-DNA hybridization results (less than 40 % relatedness value) indicated that strain NW8-21 T shoud be considered to represent a novel species of the genus Pseudonocardia, for which the name Pseudonocardia soli is proposed. The type strain is strain NW8-21 T (=BCC 58125 T =NBRC 109519 T ).