Spirosoma metallilatum sp. nov., isolated from an automotive air conditioning system

A Gram-stain-negative, aerobic, rod-shaped and yellow-pigmented bacterial strain, designated TX0405 T , was isolated from an automotive air conditioning system. Colonies were circular, convex, semi-translucent, smooth and yellow. The strain grew at 20-28°C (optimum, 28°C), at pH 6.0-7.5 (optimum, pH 6.5) and in the presence of 0-1 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strain was grouped with the members of the genus Spirosoma, with the sequence similarities of 93.0 and 92.3 % with Spirosoma panaciterrae DSM 21099 T and Spirosoma swuense JBM2-3 T , respectively. The major fatty acids of the strain were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (33.2 %), C16 : 1ω5c (25.4 %), iso-C15 : 0 (15.0 %), C16 : 0 (6.5 %) and iso-C17 : 0 3-OH (6.2 %). The predominant menaquinone was MK-7. The polar lipids were phosphatidylethanolamine, an unidentified aminolipid, two unidentified aminophospholipids and three unidentified lipids. The DNA G+C content of the type strain was 51.9 mol%. On the basis of the data presented, strain TX0405 T represents a novel species of the genus Spirosoma, for which the name Spirosomametallilatum sp. nov. (=KACC 19012 T =NBRC 112493 T ) is proposed.