Pseudooceanicola lipolyticus sp. nov., a marine alphaproteobacterium, reclassification of Oceanicola flagellatus as Pseudooceanicola flagellatus comb. nov. and emended description of the genus Pseudooceanicola

A Gram-stain-negative, rod-shaped bacterium, designated 157 T , was isolated from seawater collected from the Philippine Sea. Cells of strain 157 T grew in medium containing 0.5-10.0 % NaCl (w/v, optimum 3 %), at pH 6.0-8.5 (optimum 7.0) and at 15-40 °C (optimum 30 °C). Tweens 20, 40 and 80 as well as urea were hydrolysed. The 16S rRNA gene sequence of strain 157 T had a high sequence similarity with respect to Pseudooceanicola marinus AZO-C T (97.2 %), and exhibited less than 97.0 % sequence similarity to other type strains of the species with validly published names. Phylogenetic analyses revealed that strain 157 T fell within a cluster comprising the Pseudooceanicola species and formed a coherent clade with P. marinus AZO-C T and Pseudooceanicola antarcticus Ar-45 T . Strain 157 T exhibited average nucleotide identity values of 74.5 and 74.9 % to P. marinus LMG 23705 T and P. antarcticus Ar-45 T , respectively. In silico DNA-DNA hybridization analysis revealed that strain 157 T shared 20.2 % DNA relatedness with P. marinus LMG 23705 T and 20.6 % with P. antarcticus Ar-45 T , respectively. The sole isoprenoid quinone was ubiquinone 10. The major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C19 : 0 cyclo ω8c, C16 : 0 2-OH and C16 : 0. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content was 64.6 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, it represents a novel species of the genus Pseudooceanicola, for which the name Pseudooceanicolalipolyticus is proposed. The type strain is 157 T (=KCTC 52654 T =MCCC 1K03317 T ). In addition, the description of the genus Pseudooceanicola is emended and Oceanicola flagellatus is reclassified as Pseudooceanicola flagellatus comb. nov., with the type strain DY470 T (=CGMCC 1.12664 T =LMG 27871 T ) proposed.