Salibacterium lacus sp. nov., a halophilic, non-spore-forming bacterium isolated from sediment of a saline lake

A novel halophilic bacterium, strain GSS13 T , capable of growing at salinities of 8-28 % (w/v) NaCl (optimally at 24 %, w/v) was isolated from Yuncheng Saline Lake in China. GSS13 T was Gram-stain-positive, strictly aerobic, rod-shaped, motile and a non-spore-former. Growth occurred at pH 5.5-8.5 (optimum pH 7.0) and at 10-45 °C (optimum 30 °C). On the basis of the results of 16S rRNA gene sequences phylogenetic analyses, GSS13 T represents a member of the genus Salibacterium and is closely related to Salibacterium halotolerans S7 T , Salibacterium qingdaonense CM1 T and Salibacterium halochares MSS4 T , with 16S rRNA gene sequence similarities of 98.7, 98.4 and 97.9 %, respectively. The results of DNA-DNA pairing studies revealed that GSS13 T displayed 52, 43 and 48 % relatedness to S. halotolerans S7 T , S. qingdaonense CM1 T and S. halochares MSS4 T , respectively. The polar lipids of GSS13 consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol unidentified glycolipids, an unidentified phospholipid and an unidentified lipid. The predominant isoprenoid quinone was MK-7, and the major fatty acids were anteiso-C17 : 0 (32.0 %) and anteiso C15 : 0 (26.4 %). The DNA G+C content of the type strain was 52.1 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, a novel species of the genus Salibacterium is proposed, with the name Salibacterium lacus sp. nov. The type strain is GSS13 T (=KCTC 33792 T =MCCC 1K00567 T ).