Dyella agri sp. nov., isolated from reclaimed grassland soil

A novel strain, DKC-1 T , was isolated from reclaimed grassland soil and was characterized taxonomically by a polyphasic approach. Strain DKC-1 T was a Gram-staining-negative, light-yellow-coloured and rod shaped bacterium, motile with polar flagellum. It was able to grow at 20-37 °C, at pH 4.5-9.0 and with 0-3 % (w/v) NaCl concentration. Based on the 16S rRNA gene sequence analysis, strain DKC-1 T formed a clade within the members of the genus Dyella and showed highest sequence similarities to Dyella japonica XD53 T (98.36 %), Rhodanobacter aciditrophus sjH1 T (97.92 %), Rhodanobacter koreensis THG-DD7 T (97.74 %), Dyella kyungheensis THG-B117 T (97.65 %) and Rhodanobacter terrae GP18-1 T (97.40 %). The only respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidyl-N-methylethanolamine. The predominant fatty acids of strain DKC-1 T were iso-C16 : 0, iso-C15 : 0, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), iso-C17 : 0, iso-C11 : 0 3-OH and iso-C11 : 0. The genomic DNA G+C content of this novel strain was 63.1 mol%. The DNA-DNA relatedness between strain DKC-1 T and its reference strains (D. japonica XD53 T , R. aciditrophus sjH1 T , R. koreensis THG-DD7 T , D. kyungheensis THG-B117 T and R. terrae GP18-1 T ) was 52.3, 44.7, 38.7, 49.0 and 32.7 %, respectively, which falls below the threshold value of 70 % for the strain to be considered as novel. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain DKC-1 T represents a novel species of the genus Dyella, for which the name Dyella agri sp. nov. is proposed. The type strain is DKC-1 T (=KEMB 9005-571 T =KACC 19176 T =JCM 31925 T ).