Paracoccus sordidisoli sp. nov., isolated from an agricultural field contaminated with hexachlorocyclohexane isomers

A novel bacterial strain, designated LP91, was isolated from an agricultural field contaminated with hexachlorocyclohexane (HCH) isomers at Ummari Village, Lucknow, Uttar Pradesh, India. Cells of the strain were aerobic, short rod or coccoid, Gram-stain-negative and non-motile. Colonies of the strain were initially transparent but with time changed to a creamy white colour. Phylogenetic analysis based on the 16S rRNA marker gene showed that it was closely associated with Paracoccus aestuariivivens GHD-30 (99.1 %) and Paracoccus limosus NB88 (98.0 %), followed by Paracoccus laeviglucosivorans 43P (97.9 %) and Paracoccus marinus KKL-A5 (97.0 %). The DNA-DNA hybridization values of strain LP91 with the closely related type strains mentioned above were below 51.2±0.64 %, confirming it as a distinct species from other known species of the genus Paracoccus. The major cellular fatty acids of strain LP91 were C18 : 0 ω7c/C18 : 0 ω6c and C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and aminophospholipid, along with other lipids including glycolipids, aminolipids and other unknown phosphoglycolipids. Spermine was the major polyamine, along with putrescine in a minor amount. Ubiquinone (Q-10) was the sole isoprenoid quinone. Based on the results of phylogenetic, phenotypic and chemotaxonomic analysis, it is proposed that the isolate represents a new species of the genus Paracoccus, for which the name Paracoccus sordidisoli sp. nov. is proposed. The type strain is LP91 (=KCTC 42938=CCM 8696=MCC 3128).