Massilia glaciei sp. nov., isolated from the Muztagh Glacier

A Gram-stain-negative, rod-shaped, bacterial strain, B448-2 T , was isolated from an ice core from the Muztagh Glacier, on the Tibetan Plateau. B448-2 T grew optimally at pH 7.0 and 20 °C in the presence of 0-1.0 % (w/v) NaCl. The results of 16S rRNA gene sequence similarity analysis indicated that B448-2 T was closely related to Massilia eurypsychrophila CGMCC 1.12828 T , Rugamonas rubra CCM3730 T and Duganella zoogloeoides JCM20729 T at levels of 97.8, 97.7  and 97.3 %, respectively. The predominant fatty acids of B448-2 T were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The predominant isoprenoid quinone was Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content of the strain was 66.1 mol%. In DNA-DNA hybridization tests, B448-2 T shared 37.6 % DNA-DNA relatedness with Massilia eurypsychrophila CGMCC 1.12828 T . On the basis of the results for phenotypic and chemotaxonomic characteristics, B448-2 T was considered to represent a novel species of the genus Massilia, for which the name Massiliaglaciei sp. nov. is proposed. The type strain is B448-2 T (=JCM 30271 T =CGMCC 1.12920 T ).