Bifidobacterium vansinderenii sp. nov., isolated from faeces of emperor tamarin (Saguinus imperator)

A novel Bifidobacterium strain, Tam10B T , i.e. LMG 30126 T , was isolated from emperor tamarin (Saguinus imperator). Cells were Gram-positive, non-motile, non-sporulating, non-haemolytic, facultative anaerobic and fructose 6-phosphate phosphoketolase-positive. Phylogenetic analyses based on 16S rRNA genes as well as multilocus sequences (representing hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that Bifidobacterium Tam10B T exhibited close phylogenetic relatedness to Bifidobacterium tissieri DSM 100201 T . Comparative analysis of 16S rRNA gene sequences confirmed the phylogenetic results showing the highest gene sequence identity with strain B. tissieri DSM 100201 T (96.5 %). Furthermore, genotyping based on the genome sequence of Tam 10B, in combination with phenotypic analyses, clearly showed that strain Tam10B T is distinct from each of the type strains of the so far recognized Bifidobacterium species. The type strain Tam10B T (=LMG 30126 T =CCUG 70655 T ) represents a novel species, for which the name Bifidobacteriumvansinderenii sp. nov is proposed.