Ramlibacter rhizophilus sp. nov., isolated from rhizosphere soil of national flower Mugunghwa from South Korea

A Gram-negative, aerobic, non-motile, long rods or coccoid without flagellum strain, designated THG-YS3.2.7 T , was isolated from therhizosphere soil of a Mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-40 °C (optimum 28-37 °C), at pH 6-8 (optimum 7) and at 0-5 % NaCl (optimum 1 %). The predominant ubiquinone was ubiquinone 8 (Q-8). The major cellular fatty acids were C10 : 0, C10 : 0 3OH, C16 : 0, C17 : 0, C17 : 0 cyclo, C18 : 0, C18 : 3ω6c (6,9,12), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1ω6c). The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC). The DNA G+C content of strain THG-YS3.2.7 T was 69.4 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.2.7 T were identified as Ramlibacter henchirensis DSM 14656 T (97.92 %), Ramlibacter tataouinensis DSM 14655 T (97.90 %), Ramlibacter solisilvae KACC 17567 T (97.04 %). DNA-DNA hybridization values between strain THG-YS3.2.7 T and R. henchirensis DSM 14656 T , R. tataouinensis DSM 14655 T , R. solisilvae KACC 17567 T were 32.5±1.5, 43.1±1.1, 42.8±1.1 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-YS3.2.7 T represent a novel species of the genus Ramlibacter, for which the name Ramlibacter rhizophilus sp. nov. is proposed. The type strain is THG-YS3.2.7 T (=KCTC 52083 T =CCTCC AB 2015357 T ).