Halofilum ochraceum gen. nov., sp. nov., a gammaproteobacterium isolated from a marine solar saltern

A Gram-stain-negative, oxidase-negative, catalase-positive, facultative anaerobe, designated XJ16T, was isolated from a marine solar saltern on the coast of Weihai, China. Cells of strain XJ16T were long and rod-shaped. The colonies were ochre in colour and were able to reduce nitrate to nitrite. Optimal growth occurred at 33-37 °C (range, 20-45 °C) and in the presence of 8-10 % (w/v) NaCl (range, 2-20 %). The pH range for growth was found to be 6.5-9.5, with optimum growth at pH 7.5-8.0. Phylogenetic analysis based on the 16S rRNA gene sequence demonstrated that strain XJ16T was related to the phylum Proteobacteria. The most closely related neighbours were species of the genus Thioalkalivibrio, and the 16S rRNA gene sequence of strain XJ16T shared 93.1 % similarity with that of Thioalkalivibrio sulfidiphilus HL-EbGr7T and 93.0 % similarity with that of Thioalkalivibrio denitrificans ALJDT. The G+C content of the genomic DNA was 65.9 mol% (HPLC). The sole respiratory quinone was Q-8, and the predominant cellular fatty acids (>10 %) were iso-C15 : 0 2-OH/C16 : 1ω7c, C18 : 0 and C16 : 0 10-CH3. The predominant polar lipids in strain XJ16T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. Based on these phylogenetic, physiological and biochemical characteristics, strain XJ16T should be classified representing a novel species of a new genus within the family Ectothiorhodospiraceae, for which the name Halofilum ochraceum gen. nov., sp. nov. is proposed. The type strain of the type species is XJ16T (=KCTC 42259T=MCCC 1H00120T=CICC 23817T).