Massilia pinisoli sp. nov., isolated from forest soil

A Gram-stain-negative, aerobic, motile with a single polar flagellum, non-spore-forming and rod-shaped bacterial strain named T33T was isolated from forest soil collected at Kyonggi University, South Korea. The strain was catalase- and oxidase-positive, colonies grew on R2A agar at 32 °C. Sequencing of the 16S rRNA gene and phylogenetic analysis revealed that T33T represented a member of the genus Massilia and is closely related to Massilia niastensis KACC 12599T (98.7 % sequence similarity), Massilia aerilata KACC 12505T (98.5 %), Massilia tieshanensis KACC 14940T (98.4 %), Massilia kyonggiensis KACC 17471T (98.1 %), Massilia norwichensis LMG 28164T (97.7 %), Massilia haematophila CCUG 38318T (97.4 %), Massilia consociata CCUG 58010T (97.3 %), and Massilia niabensis KACC 12632T (97.0 %). Ubiquinone Q-8 is the predominant respiratory quinone, and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol are the major polar lipids. The major fatty acids are summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c), and C16 : 0. DNA-DNA hybridization revealed <70 % relatedness between strain T33T and the most closely related type strains. The DNA G+C content of strain T33Tis 69.4 mol%. Based on physiological and biochemical test results, Massilia pinisoli T33T is proposed as a novel species of the genus Massilia. The type strain is T33T (=KACC 18748T=KEMB 9005-368T=JCM 31316T).