Gramella sediminilitoris sp. nov., isolated from a tidal flat

A Gram-stain-negative, aerobic, non-spore-forming, motile by gliding and coccoid, ovoid or rod-shaped bacterial strain, designated GHTF-27 T , was isolated from a tidal flat at Goheung, South Korea, and subjected to a taxonomic study using a polyphasic approach. The novel strain grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-5.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GHTF-27 T belongs to the genus Gramella. It exhibited 16S rRNA gene sequence similarity values of 97.6-98.3 % to the type strains of Gramella aestuariivivens, Gramella echinicola, Gramella gaetbulicola, Gramella aquimixticola, Gramella aestuarii and Gramella portivictoriae, respectively, and of 93.8-96.7 % to the type strains of the other species of the genus Gramellawith validly published names. Strain GHTF-27 T contained MK-6 as the predominant menaquinone and iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The major polar lipids of strain GHTF-27 T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain GHTF-27 T was 39.7 mol% and DNA-DNA relatedness values with the type strains of the six phylogenetically closely related species of the genus Gramellawere 12-28 %. The differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GHTF-27 T is separated from other species of the genus Gramella. On the basis of the data presented, strain GHTF-27 T is considered to represent a novel species of the genus Gramella, for which the name Gramella sediminilitorissp. nov. is proposed. The type strain is GHTF-27 T (=KCTC 52208 T =NBRC 111992 T ).