Flavobacterium tyrosinilyticum sp. nov., isolated from the rhizosphere of wild strawberry

A Gram-stain-negative, facultatively anaerobic, motile and rod-shaped bacterial strain, designated THG-DN8.8 T , was isolated from the rhizosphere of a wild strawberry plant, located on Gyeryong Mountain, Daejeon, Republic of Korea. According to 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Flavobacterium and was related most closely to Flavobacterium banpakuense 15F3 T (97.7 % sequence similarity), Flavobacterium ginsenosidimutans THG-01 T (97.3 %), Flavobacterium ginsengiterrae DCY55 T (96.9 %) and Flavobacterium chungbukense CS100 T (96.7 %). Catalase and oxidase tests were positive. Levels of DNA-DNA relatedness with its phylogenetically closest neighbours were below 40.0 %. The DNA G+C content was determined to be 39.1 mol%. Strain THG-DN8.8 T also was found to be able to grow at 4-33 °C, with 0-2 % (w/v) NaCl and at pH 5.5-9.5. The major fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. Menaquinone-6 (MK-6) was the dominant respiratory quinone, and phosphatidylethanolamine and phosphatidyldiethanolamine were the main polar lipids. On the basis of the data presented, strain THG-DN8.8 T represents a novel species, for which the name Flavobacterium tyrosinilyticum sp. nov. is proposed. The type strain is THG-DN8.8 T (=KCTC 42726 T =CCTCC AB 2015295 T ).