Domibacillus antri sp. nov., isolated from the soil of a cave

A Gram-reaction-positive, strictly aerobic, capsule-forming, motile and rod-shaped bacterium, designated strain XD80 T , was isolated from the soil of a native cave in Lichuan, Hubei province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XD80 T was most closely related to Domibacillus iocasae CCTCC AB 2015183 T (98.66 % sequence similarity), followed by Domibacillus robiginosus DSM 25058 T (97.83 %), Domibacillus tundrae KCTC 33549 T (97.70 %), Domibacillus enclensis CCTCC AB 2011121 T (97.21 %) and Domibacillus indicus DSM 28032 T (96.96 %). Levels of DNA-DNA relatedness between strain XD80 T and D. iocasae CCTCC AB 2015183 T , D. robiginosusDSM 25058 T , D. tundrae KCTC 33549 T and D. enclensis CCTCC AB 2011121 T were 37.4 %, 53.8 %, 53.6 % and 52.7 %, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipid and two unknown phospholipids. The predominant fatty acids (>5 %) were iso-C15 : 0 (37.3 %), anteiso-C15 : 0 (10.8 %), C16 : 0 (10.4 %), iso-C17 : 0 (10.3 %), C16 : 1ω11c (9.6 %) and anteiso-C17 : 0 (7.4 %). MK-6 (86.4 %) was the major respiratory quinone. The DNA G+C content was 46.4 mol%. The cell-wall peptidoglycan contained meso-diaminopimelic acid (type A1γ). Ribose and glucose were the major whole-cell sugars. In addition, strain XD80 T showed differential physiological characteristics from most members of the genus Domibacillus, encompassing hydrolysis of starch, acid production from inositol and raffinose, and production of valine arylamidase. The results of this polyphasic study indicated that strain XD80 T represents a novel species of the genus Domibacillus, for which the name Domibacillus antri sp. nov. is proposed. The type strain is XD80 T (=CCTCC AB 2015053 T =KCTC 33636 T ).