Pedobacter humicola sp. nov., a member of the genus Pedobacter isolated from soil

An aerobic, Gram-stain-negative, oxidase-negative, catalase-positive, non-motile, non-spore-forming, rod-shaped, light pink-pigmented bacterium, designated strain R135T, was isolated from soil in Hwaseong, South Korea. Flexirubin-type pigments were absent. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain R135T formed a lineage within the family Sphingobacteriaceae of the phylum Bacteroidetes. It was distinct from various species of the genus Pedobacter, including P. terrae DS-57T (98.13 % sequence similarity), P. alluvionis NWER-II11T (97.76 %), P. suwonensis 15-52T (97.71 %), P. kyungheensis KACC 16221T (97.37 %), P. roseus CG-GP80T (97.24 %), P. soli 15-51T (97.23 %) and P. sandarakinus DS-27T (97.09 %). The major isoprenoid quinone was menaquinone-7 (MK-7), and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0, iso-C17 : 0 3-OH and C16 : 0. The DNA G+C content of strain R135T was 40.4 mol%. Levels of DNA-DNA hybridization similarities between strain R135Tand other members of the genus Pedobacter ranged from 25 % to 43 %. On the basis of phenotypic, genotypic and phylogenetic analysis, strain R135T represents a novel species of the genus Pedobacter, for which the name Pedobacter humicola sp. nov. is proposed. The type strain is R135T (=KEMB 9005-332T=KACC 18452T=JCM 31010T).