Marinobacterium profundum sp. nov., a marine bacterium from deep-sea sediment

A Gram-stain-negative, rod-shaped and motile strain, designated PAMC 27536 T , was isolated from deep-sea sediment in the East Sea, Korea. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Marinobacterium . Phylogenetic analyses revealed that strain PAMC 27536 T was related most closely to Marinobacterium rhizophilum CL-YJ9 T with a 16S rRNA gene sequence similarity of 98.5 % and to other members of the genus Marinobacterium (94.0-91.7 %). Genomic relatedness analyses between strain PAMC 27536 T and M. rhizophilum KCCM 42386 T gave an average nucleotide identity of 85.6 % and an estimated DNA-DNA hybridization of 24.6 % using the genome-to-genome distance calculator, indicating that they represent genomically distinct species. Cells of strain PAMC 27536 T grew optimally at 25-30 °C and pH 7.0-7.5 in the presence of 3 % (w/v) sea salts. The major cellular fatty acids were C 16 : 1 ω6 c and/or C 16 : 1 ω7 c , C 18 : 1 ω6 c and/or C 18 : 1 ω7 c , and C 16 : 0 . The major isoprenoid quinone was Q-8. The genomic DNA G+C content was 56.1-57.2 mol%. Based on the phylogenetic, chemotaxonomic, genomic and phenotypic data presented, a novel species with the name Marinobacterium profundum sp. nov. is proposed, with PAMC 27536 T ( = KCCM 43095 T  = JCM 30410 T ) as the type strain.