Lysobacter terricola sp. nov., isolated from greenhouse soil

Strain 5GH22-11 T , which was isolated from greenhouse soil in the Yangpyeong region, Gyeonggi province, Republic of Korea, was characterized to be an aerobic, Gram-stain-negative, flagellated, rod-shaped bacterium. It could grow at temperatures from 10 to 33 °C (optimum of 28-30 °C), in the pH range of 6.0-10.0 (optimum of pH 7.0) and without NaCl. 16S rRNA gene sequence analysis showed that strain 5GH18-14 T showed the highest sequence similarities with Lysobacter niabensis GH34-4 T (98.6 %), Lysobacter yangpyeongensis GH19-3 T (98.1 %), ' Lysobacter fragariae ' THG-DN8.7 (97.9 %), Lysobacter terrae THG-A13 T (97.3 %), ' Lysobacter rhizosphaerae ' THG-DN8.3 (97.2 %), ' Lysobacter tyrosinelyticus ' THG-DN8.2 (97.2 %) and Lysobacter oryzae YC6269 T (97.2 %), revealing less than 95.5 % sequence similarities with all other species with validly published names. Phylogenetic trees also indicated that strain 5GH18-14 T formed a compact subcluster with L. niabensis GH34-4 T , L. yangpyeongensis GH19-3 T , L. terrae THG-A13 T and L. oryzae YC6269 T within the genus Lysobacter . The predominant quinone of strain 5GH18-14 T was Q-8. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylmonomethylethanolamine in large amounts, and moderate or small amounts of three unknown phospholipids and two unknown aminophospholipids. DNA-DNA hybridization values with closely related species were below 70 %. The DNA G+C content was 65.9 mol%. Based on the phylogenetic, physiological and chemotaxonomic data, it has been demonstrated that strain 5GH18-14 T represents a novel species of the genus Lysobacter , for which the name Lysobacter terricola sp. nov. is proposed. The type strain is 5GH18-14 T ( = KACC 16954 T  = JCM 30862 T ).