Pseudahrensia todarodis sp. nov., isolated from the gut of a Japanese flying squid, Todarodes pacificus

A novel Gram-stain-negative, non-motile, non-spore-forming, non-flagellated, aerobic, beige-coloured and rod-shaped bacterium, designated strain KHS02 T , was isolated from the intestinal tract of a Japanese flying squid, Todarodes pacificus , which was collected from the East Sea, Korea. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain KHS02 T formed a monophyletic clade with Pseudahrensia aquimaris HDW-32 T , with which it had the highest sequence similarity (98.67 %). Strain KHS02 T grew optimally at pH 7 with 2 % (w/v) NaCl at 25 °C on marine broth 2216, but could not grow without Na + . The predominant isoprenoid quinone was ubiquinone-10 (Q-10). The major fatty acids (>10 % of the total) were summed feature 8 (C 18 : 1 ω6 c and/or C 18 : 1 ω7 c ) and 11-methyl C 18 : 1 ω7 c . The polar lipids of strain KHS02 T comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid and an unidentified lipid. The genomic DNA G+C content was 58.6 mol%. DNA-DNA hybridization showed that the isolate shared 16.2 ± 1.3 % (reciprocal, 15.7 ± 2.8 %) genomic relatedness with the type strain of the closest species. In conclusion, this isolate is suggested to represent a novel species of the genus Pseudahrensia , for which the name Pseudahrensia todarodis is proposed. The type strain is KHS02 T ( = KACC 18257 T  = JCM 30419 T ).