Pseudofulvibacter marinus sp. nov., isolated from seawater

A novel Gram-stain-negative, rod-shaped, non-spore-forming, non-flagellated, strictly aerobic strain, designated RZW2-1 T , was isolated from coastal seawater of the Yellow Sea in China (35.475° N 119.613° E). The organism grew optimally at 24 °C, at pH 7.0 and in the presence of 3.0 % (w/v) NaCl. The strain requires seawater or artificial seawater for growth and NaCl alone does not support growth. Strain RZW2-1 T contained MK-6 as the only respiratory quinone and iso-C 15 : 0 , iso-C 15 : 1 G and 10-methyl C 16 : 0 and/or iso-C 17 : 1 ω9 c as the dominant fatty acids. The polar lipids of strain RZW2-1 T were four unidentified phospholipids (PL1-PL4), two unknown lipids (L1, L2) and one unidentified aminolipid (AL1). The DNA G+C content of strain RZW2-1 T was 32 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was most closely related to the type strain of the only described species of genus Pseudofulvibacter , Pseudofulvibacter geojedonensis YCS-9 T , with 95.1 % 16S rRNA gene sequence similarity. On the basis of polyphasic analyses, strain RZW2-1 T represents a novel species of the genus Pseudofulvibacter , for which the name Pseudofulvibacter marinus sp. nov. is proposed. The type strain is RZW2-1 T ( = JCM 30826 T  = MCCC 1K00695 T ).