Pseudoclavibacter endophyticus sp. nov., isolated from roots of Glycyrrhiza uralensis

A Gram-stain-positive, aerobic, rod-shaped, non-motile actinomycete strain, designated EGI 60007 T , was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Yili County, Xinjiang Province, north-west China. A polyphasic approach was applied to study the taxonomic position of the new isolate. The 16S rRNA gene sequence of strain EGI 60007 T had highest similarities with members of the genus Pseudoclavibacter , including Pseudoclavibacter chungangensis CAU 59 T (96.98 % 16S rRNA gene sequence similarity), Pseudoclavibacter helvolus DSM 20419 T (96.43 %) and Pseudoclavibacter terrae THG-MD12 T (96.14 %). The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain EGI 60007 T clustered with members of the genus Pseudoclavibacter , and formed a distinct clade with P. chungangensis CAU 59 T . The polar lipids detected for strain EGI 60007 T were phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified lipid. The DNA G+C content was determined to be 63.3 mol%. The chemotaxonomic features of strain EGI 60007 T showed typical characteristics of the genus Pseudoclavibacter , with MK-9 as the respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan, and anteiso-C 15 : 0 , anteiso-C 17 : 0 and iso-C 16 : 0 as the major fatty acids. The sugars of whole-cell hydrolysates were mainly mannose, rhamnose, ribose and glucose, and a minor amount of xylose. Based on the results of the phylogentic analysis supported by morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60007 T is considered to represent a novel species of the genus Pseudoclavibacter , for which the name Pseudoclavibacter endophyticus sp. nov. is proposed. The type strain is EGI 60007 T ( = CGMCC 1.15081 T = KCTC 39112 T = DSM 29943 T ).