Lampropedia cohaerens sp. nov., a biofilm-forming bacterium isolated from microbial mats of a hot water spring, and emended description of the genus Lampropedia

A biofilm-forming, Gram-stain-negative, aerobic, catalase-positive but oxidase-negative strain, designated CT6 T , was isolated from the microbial mats (∼45 °C) of a hot water spring, located within the Himalayan ranges at Manikaran, Himachal Pradesh, India. Strain CT6 T formed white, smooth colonies with irregular margins. Transmission electron microscopy revealed coccoid, non-flagellated cells with wavy boundaries. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CT6 T belongs to the genus Lampropedia with a sequence similarity value of 95.4 % to the sole member of this genus, Lampropedia hyalina ATCC 11041 T . Strain CT6 T was found to have phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. The major cellular fatty acids were C 16 : 0 , summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ), C 14 : 0 , C 19 : 0 ω8 c cyclo and summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ). The major respiratory quinone was ubiquinone-8. The major polyamines were putrescine, spermidine and the betaproteobacterial-specific 2-hydroxyputrescine. The DNA G+C content was 63.5 mol%. Based on the genotypic, phenotypic, physiological and biochemical data, strain CT6 T is considered to represent a novel species of the genus Lampropedia , for which the name Lampropedia cohaerens sp. nov. is proposed ( = DSM 100029 T  = KCTC 42939 T  = MCC 2711 T ).